Primary Duties : The Yoh resource is expected to apply expertise across several practical skills in support of the Client’s Vaccines R&D Global Immunology function, including spectral flow cytometry, peripheral blood mononuclear cell (PBMC) processing, B cell staining and sorting, high-throughput immunoglobulin repertoire sequencing (both 10x Genomics and plate-based approaches), bioinformatics analysis of antibody sequences, and downstream functional assessment using various platforms.
The role is a lab-based research associate who will be participating in various teams to support preclinical and early clinical evaluation of vaccine candidates against various respiratory viruses. Knowledge of immunoassay-based instruments, including flow cytometers, cell sorters, multi-mode plate readers and plate washers will be required. Individual will be performing laboratory work including immunoassays, recording data in specified formats and/or recording detailed day-to-day work in electronic laboratory notebook; reagent preparation; lab housekeeping and maintenance. There will be requirement to work with animal and human blood, serum, plasma and other samples sources. Applicant must be capable of working both independently and as part of a structured team.
Responsibilities include but are not limited to:
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- Participate in design, planning, optimization and execution of studies to set up end-to-end B cell analytics pipeline integrating wet-lab and computational workflows.
- Participate in optimization and implementation of assays (serological and cell-based) for analysis of immune responses to vaccine candidates.
- Upon appropriate training perform technical procedures and operate a variety of lab instrumentation platforms related to Immunology.
- Perform PBMC isolation, B cell staining, and sorting (FACS) for repertoire analyses.
- Conduct immunoglobulin sequencing using both 10x Genomics and plate-based methods.
- Support library preparation, quality control, and sample tracking throughout the sequencing pipeline.
- Support bioinformatics analyses to process and interpret antibody repertoire datasets.
- Execute and analyze binding and epitope characterization assays using Carterra or comparable platforms.
- Upon training analyze and summarize experimental outcomes, record experimental procedures and raw and analyzed data in electronic laboratory notebook.
- Participate as required in team meetings, project discussions, and data review sessions.
- Comply with the company safety policies and minimize risk to self, others, and the environment, consistent with the community and industry standards.
Education and Experience Requirements:
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- BS +2-4 years or MS +1-2 years of relevant experience in industry or academia.
- Degree should be in Immunology, Virology, Molecular Biology or related field.
- Hands-on experience with RNA/DNA (deoxyribonucleic acid/ ribonucleic acid) extraction, Next-Generation Sequencing (NGS) library prep, paired BCR-transcriptomic analysis and single cell data quality control, hashtagging, single-cell sequencing workflows (experience with 10x Genomics preferred), or Octet or Carterra platforms
Key Skills, Abilities, and Competencies:
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- Basic proficiency in R/Python, experience with sequence annotation or other single cell analysis.
- Ability to perform spectral flow cytometry including 20+ color panels, knowledge of spectral unmixing, and experience with Cytek and Sony instrumentation.
- Proficiency in PBMC sample processing, flow cytometry, cell sorting, and multicolor staining of immune cells.
- Good time management and prioritization skills and attention to details.
- Prior experience in antibody discovery, or immune profiling projects would be a plus.
- Prior experience working in Bio Safety Lavel 2/2+ conditions would be a plus.
- Data review
- Training
- Scheduling
- GDP
- GRP, compliance requirements for clinical trial samples.
Estimated Min Rate: $24.50
Estimated Max Rate: $35.00
What’s In It for You?
We welcome you to be a part of the largest and legendary global staffing companies to meet your career aspirations. Yoh’s network of client companies has been employing professionals like you for over 65 years in the U.S., UK and Canada. Join Yoh’s extensive talent community that will provide you with access to Yoh’s vast network of opportunities and gain access to this exclusive opportunity available to you. Benefit eligibility is in accordance with applicable laws and client requirements. Benefits include:
- Medical, Prescription, Dental & Vision Benefits (for employees working 20+ hours per week)
- Health Savings Account (HSA) (for employees working 20+ hours per week)
- Life & Disability Insurance (for employees working 20+ hours per week)
- MetLife Voluntary Benefits
- Employee Assistance Program (EAP)
- 401K Retirement Savings Plan
- Direct Deposit & weekly epayroll
- Referral Bonus Programs
- Certification and training opportunities
Note: Any pay ranges displayed are estimations. Actual pay is determined by an applicant's experience, technical expertise, and other qualifications as listed in the job description. All qualified applicants are welcome to apply.
Yoh, a Day & Zimmermann company, is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.
Visit https://www.yoh.com/applicants-with-disabilities to contact us if you are an individual with a disability and require accommodation in the application process.
For California applicants, qualified applicants with arrest or conviction records will be considered for employment in accordance with the Los Angeles County Fair Chance Ordinance for Employers and the California Fair Chance Act. All of the material job duties described in this posting are job duties for which a criminal history may have a direct, adverse, and negative relationship potentially resulting in the withdrawal of a conditional offer of employment.
It is unlawful in Massachusetts to require or administer a lie detector test as a condition of employment or continued employment. An employer who violates this law shall be subject to criminal penalties and civil liability.
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